منابع مشابه
Field Flow Fractionation
The capillary electrophoresis assay described in this study is reproducible, more sensitive and faster than other analytical tests. The samples used in the capillary electrophoresis assay were obtained from brain and the lymphoid system of the animals. The sensitivity of this assay made it possible to test samples from other tissues that contain much less abnormal prion protein than brain sampl...
متن کاملDual-field and flow-programmed lift hyperlayer field-flow fractionation.
Field and flow programming and their combination, dual programming, are shown to extend the particle size range to which a single flow/hyperlayer field-flow fractionation (FFF) run is applicable to approximately 1-50 microns. The rationale for programming flow/hyperlayer FFF (or other forms of lift hyperlayer FFF) is to expand the diameter range of micron size particles that can be resolved in ...
متن کاملCellular Separation via Dielectrophoretic Field-Flow-Fractionation
I present a brief review of a cellular separation technique known as dielectrophoretic field-flowfractionation. The technique relies upon suspending different cells at different heights within a laminar flow profile. The differentially suspended cells, thus, travel at different speeds and elute from the flow chamber at different times resulting in successful separation. An alternate fabrication...
متن کاملDielectrophoretic field-flow fractionation of electroporated cells.
We describe the development and testing of a setup that allows for DEP field-flow fractionation (DEP-FFF) of irreversibly electroporated, reversibly electroporated, and nonelectroporated cells based on their different polarizabilities. We first optimized the channel and electrode dimensions, flow rate, and electric field parameters for efficient DEP-FFF separation of moderately heat-treated CHO...
متن کاملCell separation by dielectrophoretic field-flow-fractionation.
Dielectrophoretic field-flow-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34+ hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped ...
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ژورنال
عنوان ژورنال: Analytical and Bioanalytical Chemistry
سال: 2014
ISSN: 1618-2642,1618-2650
DOI: 10.1007/s00216-013-7603-9